来自《耶鲁大学开放课程：生物医学工程探索 第4集 第4集》

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  So you know everywhere on this picture you could write down exactly
  所以你能准确的知晓
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  what the sequence of nucleotides are that make up this vector.
  组成图上这个载体的任何一处的核酸序列
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  This region here of this particular plasmid is called
  质粒上的这个区域叫
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  the ori or origin of replication.
  ori 或者叫复制起点
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  Remember we talked about how DNA replicates itself and that there are enzymes,
  请记住DNA复制必须有酶的参与
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  DNA polymerase that bind to the double stranded DNA,
  DNA聚合酶结合在双链DNA上
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  separate it, denature it locally,
  打开双链并原位变性
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  and then start the process of replication.
  然后才开始复制
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  One of the properties that you would like a plasmid to have
  所以你所希望质粒具备的一个属性
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  is you'd like for cells to be able to replicate it,
  就是得能让细胞复制它
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  to make more copies of it.
  制造出更多的拷贝
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  That way you could deliver a small number of vectors and
  这样你只需向细胞中转入少量载体