来自《耶鲁大学开放课程：生物医学工程探索 第4集 第4集》

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  the primers that you've added automatically bind
  加入的引物就会在杂交的步骤中自动
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  through the process of hybridization,
  和解旋后的DNA结合
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  and then you turn up the temperature
  然后你再将温度升高至
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  to the optimum for Taq polymerase
  Taq酶的最适温度
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  and DNA synthesis starts.
  DNA就开始合成了
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  The polymerase starts a process of replication of your DNA sequences.
  聚合酶使DNA序列开始复制
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  You first separated your DNA,
  首先解旋DNA双链
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  let the primers bind,
  使引物结合
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  then turn on the enzyme,
  接着激活聚合酶
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  and it makes copies of each one of these.
  最终得到各链的复制产物
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  You then denature again,
  然后你可以再次使产物链变性
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  each one of these strands gets separated,
  使各链解旋