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In that case,
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we took the gene for the Hepatitis B protein.
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Took it out of the virus completely,
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cloned it into a plasmid,
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that plasmid was expressed in a foreign host,
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in this case it was expressed in yeast cells.
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Yeast cells were grown in large numbers with this plasmid inside,
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they expressed the plasmid
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and so you made Hepatitis B surface antigen
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not in people but in cell culture
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where it was not normally formed.
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Then that subunit was purified and formulated into the vaccine,